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Diagnostic kit

f-HPV™ Typing Kit

Multiplex fluorescent PCR assay designed for simultaneously detecting and directly typing 16 HPV types.

Overview

Cervical cancer is one of the most common cancers in women. A key risk factor is genital infection with human papilloma virus (HPV). HPV is among the most commonly sexually transmitted infections and there are more than 100 known HPV types. Persistent HPV infection is linked to cervical and other cancers, so testing for prevalent high risk types supports prevention and screening strategies.

The f HPV typingTM kit is a molecular diagnostic assay designed for simultaneously detecting and direct typing of 16 specific HPV types by multiplex fluorescent PCR. It uses 16 primers amplifying within E6 and E7 regions of HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68. Intended for use on Applied Biosystems ABI PRISM® sequencers, it uses a five-dye fluorescent system allowing multiplex amplification in the same tube for automated viral typing.


Workflow timing
DNA Extraction 20 min
Sample prep and DNA extraction step.
F-PCR Amplification 90 min
Multiplex amplification for target regions.
Electrophoresis and Analysis 30 min
Automated typing on compatible sequencers.
Result Same day
Typing output for reporting and follow-up.