Overview
The MEFV gene provides instructions for making pyrin, which helps keep inflammation under control. Mutations in MEFV can lead to reduced or malformed pyrin, contributing to inappropriate or prolonged inflammatory response. Fever and inflammation in the abdomen, chest, joints, or skin are signs associated with familial Mediterranean fever.
SNP DETECTIVE MEFV kit contains 4 primer pairs for amplification of exon 2-3-5-10 of the MEFV gene. 18 region-specific probes and 37 single-base-specific fluorescence labelled DNA probes are used for detection of SNPs. It uses a five-dye fluorescent system allowing multiplex amplification-ligation in the same tube for automated SNP analysis. The kit incorporates an external control with a wild-type pattern for related mutations to support performance checks and troubleshooting.