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Diagnostic kit

SNP Detective CVD-1 Panel Thrombophilia Kit

Multiplex system for detection and genotyping of clinically relevant variants linked to cardiovascular disease and thrombophilia risk.

Overview

Cardiovascular Diseases (CVDs) are common, but in many cases can be avoided. Atherosclerosis and venous thrombosis are major manifestations. Both are influenced by environmental and genetic parameters. Testing for genetic variations and adequate prophylaxis contributes to lower CVD risks.

The system allows detection and genotyping of variants including MTHFR (c.677 and c.1298), Prothrombin (Factor II) 20210, Factor V (Leiden R506Q), FXIII (A1) V34L and SERPINE1 (PAI1) -675 (4G/5G). It is intended for use on Applied Biosystems ABI PRISM® sequencers using a five-dye fluorescent system allowing multiplex amplification and ligation in the same tube for automated SNP analysis.


Workflow
Genomic DNA Extraction
Prepare genomic DNA from the sample.
Multiplex PCR Amplification
Target amplification with multiplex primer pairs.
Probe Ligation Reaction
Single-base specific probe ligation step.
Capillary Electrophoresis
Fragment analysis on compatible systems.
Data Analysis
Interpretation and reporting workflow.