Overview
Microdeletions of the Y chromosome are a frequent genetic cause of spermatogenetic failure in infertile men. The molecular diagnosis of Y chromosomal microdeletions is performed in the workup of male infertility in men with azoospermia or severe oligozoospermia.
The male-specific region of the Y chromosome (MSY) has been subdivided into AZFa, AZFb, AZFc and proximal AZFc (AZFd) regions.
This system consists of 14 primer pairs homologous to mapped sequence-tagged sites (STSs). It uses a five-dye fluorescent system for automated DNA fragment analysis, allowing multiplex amplification and electrophoresis of over 14 STSs including AMXY marker simultaneously. The kit is intended for use on Applied Biosystems ABI PRISM® genetic analyzers.