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Diagnostic kit

SNP Detective HBB Kit

HBB gene mutation detection kit designed for multiplex SNP analysis using a five-dye fluorescent system.

Overview

The HBB gene provides instructions for making beta-globin, a subunit of hemoglobin. Hemoglobin carries oxygen in red blood cells. Mutations in HBB are associated with conditions such as beta thalassemia and sickle cell anemia.

SNP DETECTIVE HBB kit contains 2 primer pairs for amplification targets on the HBB gene. 28 region-specific probes and 56 single-base-specific fluorescence labelled DNA probes are used for detection of SNPs. The kit uses a five-dye fluorescent system allowing multiplex amplification-ligation in the same tube for automated SNP analysis. It incorporates an external control with a wild-type pattern for related mutations to support reagent performance checks and troubleshooting.


Workflow
Genomic DNA Extraction
Prepare genomic DNA from the sample.
Multiplex PCR Amplification
Target amplification using kit primers.
Probe Ligation Reaction
Single-base specific probe ligation step.
Capillary Electrophoresis
Fragment analysis on compatible systems.
Data Analysis
Interpretation and reporting workflow.